Generalized recovery algorithm for 3D super-resolution microscopy using rotating point spread functions

Generalized recovery algorithm for 3D super-resolution microscopy using rotating point spread functions

Super-resolution microscopy with phase masks is a promising technique for 3D imaging and tracking. Due to the complexity of the resultant point spread functions, generalized recovery algorithms are still missing. We introduce a 3D super-resolution recovery algorithm that works for a variety of phase masks generating 3D point spread functions. A fast deconvolution process generates initial guess...

3D super-resolution using generalized sampling expansion

Using a probabilistic interpretation of Papoulis' generalized sampling theorem, an iterative algorithm has been devised for 3D reconstruction of a Lam-bertian surface at sub-pixel accuracy. The problem has been formulated as an optimization one in a Bayesian framework. The latter allows for introducing a priori information on the solution, using Markov Random Fields (MRF). The estimated 3D feat...

Super-resolution method using sparse regularization for point-spread function recovery

In large-scale spatial surveys, such as the forthcoming ESA Euclid mission, images may be undersampled due to the optical sensors sizes. Therefore, one may consider using a super-resolution (SR) method to recover aliased frequencies, prior to further analysis. This is particularly relevant for point-source images, which provide direct measurements of the instrument point-spread function (PSF). ...

Algorithm for 3d Localization Microscopy Using Realistic Modeling of the Point Spread Function by Phase Retrieval

Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.

Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules...